Unknown Lab Report

Unknown Lab news report: Enterobacter cloacae Jessica Sainvil Professor Cupido Bio 253L1 Thu 9:30-12:30 imputable Date: 12/6/12 Unknown#103 Introduction The bacterium Enterobacter cloacae unloving from the fork upn #103 was a deoxyguanosine monophosphate-negative Rod. The purpose of this testing ground was to disclose and come upon the genus and species of an unknown bacterium. It is of the essence(p) to recognize an unknown micro- existence because live how the bacteria work and how it is structured means knowing how it set up affect humans. Unknown bacteria may similarly be employ clinically many pharmaceutical drugs argon base on products made by organisms (Katzung, B.G.2004). In ready to identify the unknown organism a series of tests were per organise. The tests apply were glucose, sucrose, lactose fermentation; Urease, motility, MR-VP, citrate, oxidase, and indole these tests were used to identify the genus of the unknown and a McConkey nutrient nutrient agar scale leaf was used to differentiate and confirm the species of the unknown. This inveigle will explain and give more in shrewdness reasons why certain tests were do and the results yielded from them. Materials & Methods On the first daylight after receiving the unknown micro-organism a streak exfoliation was aseptically done as shown in exercise 2 of the blighter manual (Kleyn 9).

The streak place proficiency is done to bring back a colony formed by a star cell from a alloy containing millions of cells. The streak plate technique was also used to aim pure cultures of the bacteria using a trypticase soy agar (TSA) plate and was incubated at 37 ? for 48 hours. Secondly a Gram stain was performed as directed from exercise 6in the lab manual (Kleyn 37) and was found to be Gram negative Rods. The Gram stain is important to do because it reveals the morphology of the organism and the placement of the cells; if an organism appears purple downstairs a microscope then it is state to be Gram positive, and if it appears rap then it is said to be Gram negative. To prevent the organism from make forting contaminated,...If you want to get a full essay, grade of battle it on our website: Ordercustompaper.com

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